Human Semaphorin 6B - SEMA6B ELISA Kit

Mfr.No.

E1325Hu

Sensitivity

0.039ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

SEMA 6B;Sema Z;SEMA6B;Semaphorin-6B;Semaphorin-Z

Molecular Weight

95285

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1325Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.4	0.54	5.7
Intra-Assay	2	18	17.4	0.45	2.6
Intra-Assay	3	18	2.2	0.16	7

Background

Functions as a cell surface repellent for mossy fibers of developping neurons in the hippocampus where it plays a role in axon guidance. May function through the PLXNA4 receptor expressed by mossy cell axons. (Microbial infection) Acts as a receptor for P.sordellii toxin TcsL in the in the vascular endothelium.

Protocol

These standard curves of E1325Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.178	2.157	2.123
	2.137
12ng/ml	1.275	1.237	1.202
	1.199
6ng/ml	0.761	0.798	0.763
	0.835
3ng/ml	0.452	0.457	0.422
	0.462
1.5ng/ml	0.229	0.2	0.165
	0.17
0ng/ml	0.031	0.035	0
	0.038

Gene ID	10501
UniProt ID	Q9H3T3

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102967	E1325Hu	48T	$320		Add to Cart
BHE12102967	E1325Hu	96T	$458		Add to Cart

Human Semaphorin 6B - SEMA6B ELISA Kit

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