Human Semaphorin 4C - SEMA4C ELISA Kit

Mfr.No.

E1534Hu

Sensitivity

3.14ng/L

Detection Range

7-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

SEMA4C;Semaphorin-4C

Molecular Weight

92623

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1534Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	240.1	9.38	3.9
Intra-Assay	2	18	638.6	25.91	4.1
Intra-Assay	3	18	110.5	4.92	4.5

Background

Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.

Protocol

These standard curves of E1534Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2	2.059	2.011
	2.118
400ng/L	1.342	1.338	1.29
	1.333
200ng/L	0.685	0.649	0.601
	0.614
100ng/L	0.335	0.349	0.301
	0.363
50ng/L	0.183	0.191	0.143
	0.198
0ng/L	0.047	0.048	0
	0.049

Gene ID	54910
UniProt ID	Q9C0C4

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103148	E1534Hu	48T	$320		Add to Cart
BHE12103148	E1534Hu	96T	$458		Add to Cart

Human Semaphorin 4C - SEMA4C ELISA Kit

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