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Home / ELISA Kit

Human Semaphorin 3F (S3F) ELISA Kit

SKU : BHE13702930

The semaphorins are a family of proteins that are involved in signaling. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62), a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.

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Mfr.No.	KTE60714
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Semaphorin 3F (S3F) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3F.
Alternative Names	SEMA3F; SEMA-IV; SEMA4; SEMAK; sema IV; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; 3F; semaphorin 3F; semaphorinI/F; semaphorin IV
Detection Method	Colorimetric
Kit Components	Human Semaphorin 3F microplate Human Semaphorin 3F standard Human Semaphorin 3F detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Semaphorin 3F (S3F) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA3F. No significant cross-reactivity or interference between Human SEMA3F and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The semaphorins are a family of proteins that are involved in signaling. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62), a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.
Notes	This Human Semaphorin 3F (S3F) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3F in samples. An antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6405

Applications	ELISA
Applications Notes	This Human Semaphorin 3F (S3F) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3F in samples. An antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702930	KTE60714	48 T	$359		Add to Cart
BHE13702930	KTE60714	96 T	$619		Add to Cart
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