Human Semaphorin 3C - SEMA3C ELISA Kit

Mfr.No.

E1949Hu

Sensitivity

10.23ng/L

Detection Range

20-3500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Semaphorin 3C (also known as Sema3C) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Sema E;SEMA3C;Semaphorin-3C;Semaphorin-E

Molecular Weight

85207

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1949Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1248	45.84	3.7
Intra-Assay	2	18	447	22.27	5
Intra-Assay	3	18	768.9	30.84	4

Background

Binds to plexin family members and plays an important role in the regulation of developmental processes. Required for normal cardiovascular development during embryogenesis. Functions as attractant for growing axons, and thereby plays an important role in axon growth and axon guidance (By similarity).

Protocol

These standard curves of E1949Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.045	2.155	2.101
	2.265
1000ng/L	1.47	1.421	1.367
	1.372
500ng/L	0.563	0.57	0.516
	0.577
250ng/L	0.425	0.423	0.369
	0.422
125ng/L	0.139	0.174	0.12
	0.209
0ng/L	0.052	0.054	0
	0.056

Gene ID	10512
UniProt ID	Q99985

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103512	E1949Hu	48T	$320		Add to Cart
BHE12103512	E1949Hu	96T	$458		Add to Cart

Human Semaphorin 3C - SEMA3C ELISA Kit

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