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Home / ELISA Kit

Human Semaphorin 3A (SEMA3A) ELISA Kit

SKU : BHE13702933

SEMA3A is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.The first of the CRMP proteins, CRMP2, was identified as an intracellular messenger required for the growth cone-collapse induced by semaphorin 3A.

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Mfr.No.	KTE60717
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Semaphorin 3A (SEMA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3A.
Alternative Names	SEMA3A; Hsema-I; Hsema-III; MGC133243; SEMA1; SEMAD; SEMAIII; SEMAL; SemD; coll-1; OTTHUMP00000208884; collapsin 1; semaphorin 3A; semaphorin D; semaphorinI; semaphorin L; semaphorin-like
Detection Method	Colorimetric
Kit Components	Human Semaphorin 3A microplate Human Semaphorin 3A standard Human Semaphorin 3A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Semaphorin 3A (SEMA3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human SEMA3A. No significant cross-reactivity or interference between Human SEMA3A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SEMA3A is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.The first of the CRMP proteins, CRMP2, was identified as an intracellular messenger required for the growth cone-collapse induced by semaphorin 3A.
Notes	This Human Semaphorin 3A (SEMA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3A in samples. An antibody specific for SEMA3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10371

Applications	ELISA
Applications Notes	This Human Semaphorin 3A (SEMA3A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3A in samples. An antibody specific for SEMA3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702933	KTE60717	48 T	$339		Add to Cart
BHE13702933	KTE60717	96 T	$589		Add to Cart
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