Human Semaphorin 3A(Sema 3A)ELISA Kit

Mfr.No.

CSB-E15913h

Alternative Names

sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, Hsema-I, Hsema-III, MGC133243, SEMA1, SEMAD, SEMAIII, SEMAL, SemD, coll-1, OTTHUMP00000208884, collapsin 1, semaphorin 3A, semaphorin D, semaphorin III, semaphorin L, semaphorin-like

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human IGFBP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	86-97
EDTA plasma (n=4)	93	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	84-95
1:200	Average %	105
	Range %	97-108
1:400	Average %	94
	Range %	88-97
1:800	Average %	98
	Range %	94-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.613	2.557	2.585	2.493
5	1.804	1.818	1.811	1.719
2.5	0.978	0.966	0.972	0.880
1.25	0.561	0.584	0.573	0.481
0.625	0.324	0.304	0.314	0.222
0.312	0.251	0.234	0.243	0.151
0.156	0.143	0.135	0.139	0.047
0	0.091	0.093	0.092

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q14563
Antigen	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

Applications

ELISA

Research Area

SEMA3A

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504260	CSB-E15913h	96T	$695	Add to Cart
BHE10504260	CSB-E15913h	5x96T	$3336	Add to Cart
BHE10504260	CSB-E15913h	10x96T	$6602.50	Add to Cart

Human Semaphorin 3A(Sema 3A)ELISA Kit

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