Matrices listed below were spiked with certain level of SEMA5A and the recovery rates were calculated by comparing the measured value to the expected amount of SEMA5A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
96
EDTA plasma(n=5)
88-101
95
heparin plasma(n=5)
89-93
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SEMA5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SEMA5A/Semaphorin-5A/Semaphorin-F/Sema F/SEMAF/semaphorin F/SEMF/transmembrane domain(TM) and short cytoplasmic domain,(semaphorin) 5A
Notes
For Research Use Only
UniProt ID
Q13591
Antigen
SEMA5A
Applications
ELISA
Applications Notes
SEMA5A ELISA Kit allows for the in vitro quantitative determination of SEMA5A concentrations in serum, plasma, tissue homogenates and other biological fluids.