Human Selenoprotein W - SEPW1 ELISA Kit

Mfr.No.

E3477Hu

Sensitivity

2.29ng/ml

Detection Range

5-1000ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Selenoprotein W (also known as SEPW1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Selenoprotein W;selW;SEPW1

Molecular Weight

9448

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3477Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	207.24	8.29	4
Intra-Assay	2	18	326.67	7.21	2.21
Intra-Assay	3	18	175.94	6.22	3.53

Background

Plays a role as a glutathione (GSH)-dependent antioxidant. May be involved in a redox-related process. May play a role in the myopathies of selenium deficiency (By similarity).

Protocol

These standard curves of E3477Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/ml	2.282	2.248	2.197
	2.213
320ng/ml	1.341	1.395	1.344
	1.448
160ng/ml	0.638	0.655	0.605
	0.672
80ng/ml	0.329	0.326	0.276
	0.324
40ng/ml	0.141	0.147	0.097
	0.154
0ng/ml	0.056	0.05	0
	0.044

Gene ID	6415
UniProt ID	P63302

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104983	E3477Hu	48T	$320		Add to Cart
BHE12104983	E3477Hu	96T	$458		Add to Cart

Human Selenoprotein W - SEPW1 ELISA Kit

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