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Home / ELISA Kit

Human Selenium binding protein 1 (SELENBP1) ELISA Kit

SKU : BHE13702936

protein belongs to the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. It has been proposed that the effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins. The exact function of this gene is not known.
Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients.

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Mfr.No.	KTE60720
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Selenium binding protein 1 (SELENBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SELENBP1.
Alternative Names	SELENBP1; RP11-126K1.7; FLJ13813; LPSB; SP56; hSBP; hSP56;
Detection Method	Colorimetric
Kit Components	Human Selenium binding protein 1 microplate Human Selenium binding protein 1 standard Human Selenium binding protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Selenium binding protein 1 (SELENBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SELENBP1. No significant cross-reactivity or interference between Human SELENBP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	protein belongs to the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. It has been proposed that the effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins. The exact function of this gene is not known. Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients.
Notes	This Human Selenium binding protein 1 (SELENBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SELENBP1 in samples. An antibody specific for SELENBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELENBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELENBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELENBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	8991

Applications	ELISA
Applications Notes	This Human Selenium binding protein 1 (SELENBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SELENBP1 in samples. An antibody specific for SELENBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELENBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELENBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELENBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702936	KTE60720	48 T	$359		Add to Cart
BHE13702936	KTE60720	96 T	$619		Add to Cart
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BHE13702936	KTE60720	96 Tx50	$26209		Add to Cart

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