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Home / ELISA Kit

Human Secretin (SCT) ELISA Kit

SKU : BHE13702950

Secretin is a hormone that controls the secretions into the duodenum, and also separately, water homeostasis throughout the body. Acting as an anti-Müllerian hormone it is produced in the S cells of the duodenum in the crypts of Lieberkühn. Its effect is to regulate the pH of the duodenal contents via the control of gastric acid secretion and buffering with bicarbonate. It is notable for being the first hormone to be identified. In humans, the secretin peptide is encoded by the SCT gene. It has recently been discovered to play a role in osmoregulation in the hypothalamus, pituitary, and kidney.
Secretin is a linear peptide hormone, which is composed of 27 amino acids and has a molecular weight of 3055. A helix is formed in the amino acids between positions 5 and 13.

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Mfr.No.	KTE60734
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Secretin (SCT) ELISA Kit employs a two-site sandwich ELISA to quantitate SCT.
Alternative Names	SCT
Detection Method	Colorimetric
Kit Components	Human Secretin microplate Human Secretin standard Human Secretin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Secretin (SCT) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCT. No significant cross-reactivity or interference between Human SCT and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Secretin is a hormone that controls the secretions into the duodenum, and also separately, water homeostasis throughout the body. Acting as an anti-Müllerian hormone it is produced in the S cells of the duodenum in the crypts of Lieberkühn. Its effect is to regulate the pH of the duodenal contents via the control of gastric acid secretion and buffering with bicarbonate. It is notable for being the first hormone to be identified. In humans, the secretin peptide is encoded by the SCT gene. It has recently been discovered to play a role in osmoregulation in the hypothalamus, pituitary, and kidney. Secretin is a linear peptide hormone, which is composed of 27 amino acids and has a molecular weight of 3055. A helix is formed in the amino acids between positions 5 and 13.
Notes	This Human Secretin (SCT) ELISA Kit employs a two-site sandwich ELISA to quantitate SCT in samples. An antibody specific for SCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6343

Applications	ELISA
Applications Notes	This Human Secretin (SCT) ELISA Kit employs a two-site sandwich ELISA to quantitate SCT in samples. An antibody specific for SCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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