Human Secreted Frizzled-related Protein 5

Mfr.No.

E2186Hu

Description

This sandwich kit is for the accurate quantitative detection of human Secreted Frizzled-Related Protein 5 (also known as SFRP5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Frizzled-related protein 1b;FRP-1b;SARP-3;Secreted apoptosis-related protein 3;Secreted frizzled-related protein 5;SFRP 5;SFRP5;sFRP-5

Reactivity

Human

Detection Range

0.5-100ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

35563

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.25ng/ml

Background

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Gene ID	6425
UniProt ID	Q5T4F7
Antigen	SFRP5

Research Area

Stem Cells

Protocol

These standard curves of E2186Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.521	2.476	2.444
	2.431
32ng/ml	1.324	1.403	1.371
	1.483
16ng/ml	0.933	0.881	0.848
	0.829
8ng/ml	0.518	0.498	0.465
	0.478
4ng/ml	0.194	0.22	0.187
	0.246
0ng/ml	0.036	0.033	0
	0.03

Precision

We measured random samples of E2186Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	29.1	0.91	3.1
Intra-Assay	2	18	13	0.99	7.6
Intra-Assay	3	18	18.2	1.09	6

Storage	2-8°C

Reference	Cumulative effects of exercise training and consumption of propolis on managing diabetic dyslipidemia in adult women: a single-blind, randomized, controlled trial with pre-post-intervention assessments Journal: J Physiol Sci. 2023 Aug 5;73(1):17.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103727	E2186Hu	48T	$320		Add to Cart
BHE12103727	E2186Hu	96T	$458		Add to Cart

Human Secreted Frizzled-related Protein 5 - SFRP5 ELISA Kit

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