Human Secreted Frizzled Related Protein 3

Mfr.No.

E3897Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Secreted Frizzled Related Protein 3 (also known as SFRP3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Frezzled;Fritz;Frizzled-related protein 1;FRZB;FrzB-1;Secreted frizzled-related protein 3;sFRP-3

Molecular Weight

36254

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3897Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.4	0.36	6.7
Intra-Assay	2	18	7.4	0.47	6.3
Intra-Assay	3	18	13.2	0.39	3

Background

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.

Protocol

These standard curves of E3897Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.227	2.202	2.154
	2.177
10ng/ml	1.125	1.162	1.114
	1.199
5ng/ml	0.635	0.654	0.606
	0.673
2.5ng/ml	0.493	0.494	0.446
	0.495
1.25ng/ml	0.232	0.242	0.194
	0.252
0ng/ml	0.056	0.048	0
	0.041

Gene ID	2487
UniProt ID	Q92765

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105335	E3897Hu	48T	$320		Add to Cart
BHE12105335	E3897Hu	96T	$458		Add to Cart

Human Secreted Frizzled Related Protein 3 - SFRP3 ELISA Kit

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