Human Secreted frizzled-related protein 3(FRZB) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL009008HU

Alternative Names

frizzled-related protein, FRE, FRITZ, FRP-3, FRZB-1, FRZB-PEN, FRZB1, FZRB, OS1, SFRP3, SRFP3, hFIZ, frizzled homolog-related

Reactivity

Human

Detection Range

0.47 ng/mL-30 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human THSD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-110
EDTA plasma (n=4)	93	85-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human THSD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	88
	Range %	81-95
1:40	Average %	91
	Range %	86-97
1:80	Average %	97
	Range %	91-102
1:160	Average %	102
	Range %	97-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.117 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q92765
Antigen	frizzled-related protein

Applications

ELISA

Research Area

FRZB

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10504236	CSB-EL009008HU	96T	$595	Add to Cart
BHE10504236	CSB-EL009008HU	5x96T	$2856	Add to Cart
BHE10504236	CSB-EL009008HU	10x96T	$5652.50	Add to Cart