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Home / ELISA Kit

Human Secreted frizzled-related protein 2 (SFRP2) ELISA Kit

SKU : BHE13702897

SFRP2 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. The partial human SARP1 cDNA encodes a polypeptide that is 95% identical to mouse Sarp1. When expressed in a breast adenocarcinoma cell line, mouse SARP1 and human SARP2 exhibited opposite effects on cell sensitivity to proapoptotic stimuli. Whereas cells with SARP1 had higher resistance, cells expressing SARP2 were sensitized to apoptosis induced by tumor necrosis factor and ceramide. Expression of SARP1 or SARP2 modified the intracellular levels of beta-catenin, an indicator of Wnt-frizzled protein interaction and signal transduction, suggesting that SARPs interfere with the Wnt-frizzled signaling pathway.

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Mfr.No.	KTE60681
Description	This Human Secreted frizzled-related protein 2 (SFRP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP2.
Alternative Names	SFRP2; FRP-2; SARP1; SDF-5; secreted apoptosis related protein 1
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Secreted frizzled-related protein 2 microplate Human Secreted frizzled-related protein 2 standard Human Secreted frizzled-related protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Secreted frizzled-related protein 2 (SFRP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFRP2. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SFRP2 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. The partial human SARP1 cDNA encodes a polypeptide that is 95% identical to mouse Sarp1. When expressed in a breast adenocarcinoma cell line, mouse SARP1 and human SARP2 exhibited opposite effects on cell sensitivity to proapoptotic stimuli. Whereas cells with SARP1 had higher resistance, cells expressing SARP2 were sensitized to apoptosis induced by tumor necrosis factor and ceramide. Expression of SARP1 or SARP2 modified the intracellular levels of beta-catenin, an indicator of Wnt-frizzled protein interaction and signal transduction, suggesting that SARPs interfere with the Wnt-frizzled signaling pathway.
Notes	This Human Secreted frizzled-related protein 2 (SFRP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP2 in samples. An antibody specific for SFRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	6423
Antigen	SFRP2
Tag	SFRP2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Secreted frizzled-related protein 2 (SFRP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP2 in samples. An antibody specific for SFRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702897	KTE60681	48 T	$339		Add to Cart
BHE13702897	KTE60681	96 T	$589		Add to Cart
BHE13702897	KTE60681	96 Tx5	$2629		Add to Cart
BHE13702897	KTE60681	96 Tx50	$24569		Add to Cart

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