Human Secreted Frizzled Related Protein 2

Mfr.No.

E2325Hu

Sensitivity

0.24ng/ml

Detection Range

0.5-150ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Secreted Frizzled Related Protein 2 (also known as SFRP2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FRP-2;SARP-1;Secreted apoptosis-related protein 1;Secreted frizzled-related protein 2;SFRP 2;SFRP2;sFRP-2

Molecular Weight

33490

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2325Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	16.7	0.74	4.4
Intra-Assay	2	18	30.2	1.47	4.8
Intra-Assay	3	18	40	1.27	3.2

Background

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Protocol

These standard curves of E2325Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.293	2.321	2.263
	2.349
40ng/ml	1.23	1.276	1.218
	1.322
20ng/ml	0.687	0.739	0.681
	0.791
10ng/ml	0.462	0.483	0.425
	0.504
5ng/ml	0.185	0.195	0.137
	0.205
0ng/ml	0.06	0.058	0
	0.056

Gene ID	6423
UniProt ID	Q96HF1

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103863	E2325Hu	48T	$320		Add to Cart
BHE12103863	E2325Hu	96T	$458		Add to Cart

Human Secreted Frizzled Related Protein 2 - SFRP2 ELISA Kit

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