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Home / ELISA Kit

Human Secreted frizzled-related protein 1 (SFRP1) ELISA Kit

SKU : BHE13702898

Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.
SFRP1 is a 35 kDa prototypical member of the SFRP family. It acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. It is located in a chromosomal region (8p12-p11.1) that is frequently deleted in breast cancer and is thought to harbour a tumor suppressor gene.

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Mfr.No.	KTE60682
Description	This Human Secreted frizzled-related protein 1 (SFRP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP1.
Alternative Names	SFRP1; FRP; FRP-1; FRP1; FrzA; SARP2; secreted apoptosis-related protein 2
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Secreted frizzled-related protein 1 microplate Human Secreted frizzled-related protein 1 standard Human Secreted frizzled-related protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Secreted frizzled-related protein 1 (SFRP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFRP1. No significant cross-reactivity or interference between Human SFRP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart. SFRP1 is a 35 kDa prototypical member of the SFRP family. It acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. It is located in a chromosomal region (8p12-p11.1) that is frequently deleted in breast cancer and is thought to harbour a tumor suppressor gene.
Notes	This Human Secreted frizzled-related protein 1 (SFRP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP1 in samples. An antibody specific for SFRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	6422
Antigen	SFRP1
Tag	SFRP1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Secreted frizzled-related protein 1 (SFRP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP1 in samples. An antibody specific for SFRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702898	KTE60682	48 T	$359		Add to Cart
BHE13702898	KTE60682	96 T	$619		Add to Cart
BHE13702898	KTE60682	96 Tx5	$2809		Add to Cart
BHE13702898	KTE60682	96 Tx50	$26209		Add to Cart

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