Human secondary lymphoid-tissue chemokine,SLC ELISA Kit

Mfr.No.

CSB-E04521h

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Human

Sample Type

Serum, Plasma

Assay Duration

1-5h

Alternative Names

chemokine (C-C motif) ligand 21, 6Ckine, CKb9, ECL, MGC34555, SCYA21, SLC, TCA4, Efficient Chemoattractant for Lymphocytes, beta chemokine exodus-2, exodus-2, secondary lymphoid tissue chemokine, small inducible cytokine A21, small indu

Recovery

The recovery of rat FAAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	103	98-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat FAAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	107
	Range %	103-110
1:8	Average %	89
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.130	2.031	2.081	1.968
5	1.708	1.804	1.756	1.643
2.5	1.237	1.301	1.269	1.156
1.25	0.824	0.801	0.813	0.700
0.625	0.569	0.591	0.580	0.467
0.312	0.377	0.369	0.373	0.260
0.156	0.245	0.251	0.248	0.135
0	0.111	0.114	0.113	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O00585
Antigen	chemokine (C-C motif) ligand 21

Applications	ELISA
Research Area	CCL21

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504233	CSB-E04521h	96T	$790	Add to Cart
BHE10504233	CSB-E04521h	5x96T	$3792	Add to Cart
BHE10504233	CSB-E04521h	10x96T	$7505	Add to Cart

Human secondary lymphoid-tissue chemokine,SLC ELISA Kit

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