Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine®

Mfr.No.

EK1339

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.007	0.055	0.103	0.201	0.366	0.746	1.248	1.932

Description

Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine® (96 Tests). Quantitate Human SDC1 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Syndecan-1;SYND1;CD138;SDC1;SDC;

Molecular Weight

32462 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-G254

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Syndecan-1

Subcellular Localization

Membrane; Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	632	25676	8396	676	2617	8785
Standard deviation	26.51	171.27	545.74	31.09	196.27	588.59
CV (%)	4.1%	6.6%	6.5%	4.6%	7.5%	6.7%

Protein Function

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Background

Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for intealization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Whats more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	632	571	608	604	603	21.72	3.6%
Sample 2	2595	2506	2397	2686	2546	107	4.2%
Sample 3	8396	8079	9304	8737	8629	453.89	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6382
Gene Name	SDC1
UniProt ID	P18827
Protein Name	Syndecan-1
Sequence	Belongs to the syndecan proteoglycan family.

Applications	ELISA
Research Area	Cancer,Cancer Metabolism,Cell Adhesion Proteins,Cell Type Marker,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Hematopoietic Progenitors,Invasion/Microenvironment,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lymphoid,Membrane Proteins,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Neurogenesis,Neurology Process,Neuron Marker,Neuroscience,Pathways and Processes,Signal Transduction,Stem Cells,Synapse Marker

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000933	EK1339	96 wells/kit	$499		Add to Cart

Human SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine®

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