Matrices listed below were spiked with certain level of SCUBE1 and the recovery rates were calculated by comparing the measured value to the expected amount of SCUBE1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
96
EDTA plasma(n=5)
86-104
95
heparin plasma(n=5)
88-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SCUBE1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SCUBE1 ELISA Kit allows for the in vitro quantitative determination of SCUBE1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SCUBE1(Signal peptide, CUB and EGF-like domain-containing protein 1) ELISA Kit