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Home / ELISA Kit

Human Scavenger receptor class B member 1 (SCARB1) ELISA Kit

SKU : BHE13702962

Using degenerate PCR, Calvo and Vega (1993) isolated a novel sequence closely related to both the CD36 thrombospondin/collagen receptor and to lysosomal integral membrane protein II (LIMPII). This novel gene was termed CLA1 for 'CD36 and LIMPII analogous-1.' Calvo and Vega (1993) isolated 2 alternatively spliced CLA1 cDNAs predicting proteins of 409 and 509 amino acids from a human placenta cDNA library. Calvo and Vega (1993) stated that the full-length CLA1 sequence predicts a glycoprotein having 2 transmembrane domains, 2 short cytoplasmic tails, and a large extracellular loop. They used immunofluorescence to show that the protein is found on the plasma membrane. Northern blot analysis revealed a 2.9-kb mRNA in several cell lines.

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Mfr.No.	KTE60746
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Scavenger receptor class B member 1 (SCARB1) ELISA Kit employs a two-site sandwich ELISA to quantitate SCARB1.
Alternative Names	SCARB1; CD36L1; CLA-1; CLA1; MGC138242; SR-BI; SRB1; CD36 antigen (collagen type I receptor; thrombospondin receptor)-like 1; CD36 antigen-like 1; scavenger receptor class B type 1; scavenger receptor class B
Detection Method	Colorimetric
Kit Components	Human Scavenger receptor class B member 1 microplate Human Scavenger receptor class B member 1 standard Human Scavenger receptor class B member 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Scavenger receptor class B member 1 (SCARB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCARB1. No significant cross-reactivity or interference between Human SCARB1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Using degenerate PCR, Calvo and Vega (1993) isolated a novel sequence closely related to both the CD36 thrombospondin/collagen receptor and to lysosomal integral membrane protein II (LIMPII). This novel gene was termed CLA1 for 'CD36 and LIMPII analogous-1.' Calvo and Vega (1993) isolated 2 alternatively spliced CLA1 cDNAs predicting proteins of 409 and 509 amino acids from a human placenta cDNA library. Calvo and Vega (1993) stated that the full-length CLA1 sequence predicts a glycoprotein having 2 transmembrane domains, 2 short cytoplasmic tails, and a large extracellular loop. They used immunofluorescence to show that the protein is found on the plasma membrane. Northern blot analysis revealed a 2.9-kb mRNA in several cell lines.
Notes	This Human Scavenger receptor class B member 1 (SCARB1) ELISA Kit employs a two-site sandwich ELISA to quantitate SCARB1 in samples. An antibody specific for SCARB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCARB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCARB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCARB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	949

Applications	ELISA
Applications Notes	This Human Scavenger receptor class B member 1 (SCARB1) ELISA Kit employs a two-site sandwich ELISA to quantitate SCARB1 in samples. An antibody specific for SCARB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCARB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCARB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCARB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702962	KTE60746	48 T	$339		Add to Cart
BHE13702962	KTE60746	96 T	$589		Add to Cart
BHE13702962	KTE60746	96 Tx5	$2629		Add to Cart
BHE13702962	KTE60746	96 Tx50	$24569		Add to Cart

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