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Home / ELISA Kit

Human Scavenger receptor class A member 5 (SCARA5) ELISA Kit

SKU : BHE13702963

Scara5, encoding a deduced 491-amino acid protein that shares 88% sequence identity with the 495-amino acid human ortholog. Although overall identity with Scara1 is about 25%, Scara5 and Scara1 share many features, including an N-terminal cytoplasmic domain followed by a predicted transmembrane domain, a spacer region with several putative glycosylation sites, a collagenous domain, a hinge region, and a C-terminal scavenger receptor cysteine-rich domain (SRCR). The SCARA5 protein was predicted to be trimeric, as are other collagenous transmembrane proteins, and this prediction was supported by Western blotting. Based on similarity to other members of this family, the trimer is present on the surface of the plasma membrane and anchored by the transmembrane domain.

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Mfr.No.	KTE60747
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Scavenger receptor class A member 5 (SCARA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SCARA5.
Alternative Names	SCARA5; FLJ23907; MGC45780; NET33; Tesr; scavenger receptor class A; member 5; testis expressed scavenger receptor
Detection Method	Colorimetric
Kit Components	Human Scavenger receptor class A member 5 microplate Human Scavenger receptor class A member 5 standard Human Scavenger receptor class A member 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Scavenger receptor class A member 5 (SCARA5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCARA5. No significant cross-reactivity or interference between Human SCARA5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Scara5, encoding a deduced 491-amino acid protein that shares 88% sequence identity with the 495-amino acid human ortholog. Although overall identity with Scara1 is about 25%, Scara5 and Scara1 share many features, including an N-terminal cytoplasmic domain followed by a predicted transmembrane domain, a spacer region with several putative glycosylation sites, a collagenous domain, a hinge region, and a C-terminal scavenger receptor cysteine-rich domain (SRCR). The SCARA5 protein was predicted to be trimeric, as are other collagenous transmembrane proteins, and this prediction was supported by Western blotting. Based on similarity to other members of this family, the trimer is present on the surface of the plasma membrane and anchored by the transmembrane domain.
Notes	This Human Scavenger receptor class A member 5 (SCARA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SCARA5 in samples. An antibody specific for SCARA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCARA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCARA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCARA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	286133

Applications	ELISA
Applications Notes	This Human Scavenger receptor class A member 5 (SCARA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SCARA5 in samples. An antibody specific for SCARA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCARA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCARA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCARA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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