Human Scaffold Attachment Factor B1

Mfr.No.

E3949Hu

Sensitivity

2.56ng/L

Detection Range

5-1000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

HSP27 ERE-TATA-binding protein;HSP27 estrogen response element-TATA box-binding protein;SAFB;SAF-B;SAF-B1;Scaffold attachment factor B1

Molecular Weight

102642

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3949Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	400.4	22.52	5.6
Intra-Assay	2	18	63	4.38	7
Intra-Assay	3	18	141.3	8.47	6

Background

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (PubMed: 9671816). Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription (PubMed: 12660241). Thereby acts as a negative regulator of cell proliferation (PubMed: 12660241). When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity).

Protocol

These standard curves of E3949Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.186	2.201	2.142
	2.215
320ng/L	1.541	1.546	1.488
	1.551
160ng/L	1.007	0.997	0.938
	0.986
80ng/L	0.635	0.634	0.576
	0.634
40ng/L	0.465	0.458	0.4
	0.451
0ng/L	0.059	0.058	0
	0.057

Gene ID	6294
UniProt ID	Q15424

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105383	E3949Hu	48T	$320		Add to Cart
BHE12105383	E3949Hu	96T	$458		Add to Cart

Human Scaffold Attachment Factor B1 - SAFB ELISA Kit

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