Matrices listed below were spiked with certain level of SATB2 and the recovery rates were calculated by comparing the measured value to the expected amount of SATB2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
92
EDTA plasma(n=5)
86-103
93
heparin plasma(n=5)
87-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SATB2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
SATB2 ELISA Kit allows for the in vitro quantitative determination of SATB2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human SATB2(Special AT-rich sequence-binding protein 2) ELISA Kit