Human Sam Domain and Hd Domain-containing Protein 1

Mfr.No.

E6116Hu

Alternative Names

Proline-glutamate repeat-containing protein;SAM and SH3 domain-containing protein 1;SASH 1;SASH1

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

136653

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.32ng/L

Background

Is a positive regulator of NF-kappa-B signaling downstream of TLR4 activation. It acts as a scaffold molecule to assemble a molecular complex that includes TRAF6, MAP3K7, CHUK and IKBKB, thereby facilitating NF-kappa-B signaling activation (PubMed: 23776175). Regulates TRAF6 and MAP3K7 ubiquitination (PubMed: 23776175). Involved in the regulation of cell mobility (PubMed: 23333244, PubMed: 23776175, PubMed: 25315659). Regulates lipolysaccharide (LPS)-induced endothelial cell migration (PubMed: 23776175). Is involved in the regulation of skin pigmentation through the control of melanocyte migration in the epidermis (PubMed: 23333244).

Gene ID	23328
UniProt ID	O94885
Antigen	SASH1

Research Area

Others

Protocol

These standard curves of E6116Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.362	2.398	2.336
	2.433
800ng/L	1.445	1.386	1.324
	1.326
400ng/L	0.758	0.821	0.759
	0.884
200ng/L	0.51	0.53	0.468
	0.55
100ng/L	0.267	0.294	0.233
	0.322
0ng/L	0.063	0.062	0
	0.06

Precision

We measured random samples of E6116Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1124	34.2	3
Intra-Assay	2	18	511.3	32.53	6.4
Intra-Assay	3	18	352.7	22.35	6.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107478	E6116Hu	48T	$320		Add to Cart
BHE12107478	E6116Hu	96T	$458		Add to Cart

Human Sam Domain and Hd Domain-containing Protein 1 - SAMHD1 ELISA Kit

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