Human SAA/SAA1 ELISA Kit PicoKine®

Mfr.No.

EK1544

Sensitivity

<150 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.001	0.085	0.167	0.356	0.711	1.083	1.773	2.149

Description

Human SAA/SAA1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SAA1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serum amyloid A-1 protein;SAA;Amyloid protein A;Amyloid fibril protein AA;Serum amyloid protein A (2-104);Serum amyloid protein A (3-104);Serum amyloid protein A (2-103);Serum amyloid protein A (2-102);Serum amyloid protein A (4-101);SAA1;

Molecular Weight

13532 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S20-Y122

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human SAA

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	3858	13218	48138	4235	13787	47081
Standard deviation	293.2	581.59	2406.9	393.85	868.58	2777.77
CV (%)	7.6%	4.4%	5%	9.3%	6.3%	5.9%

Protein Function

Major acute phase protein.

Background

Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	3858	3499	3839	4125	3830	222.17	5.8%
Sample 2	13218	13736	13026	14070	13512	413.57	3%
Sample 3	48138	46567	52708	53611	50256	2973.33	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6288
Gene Name	SAA1
UniProt ID	P0DJI8
Protein Name	Serum amyloid A-1 protein
Tissue Specificity	Expressed by the liver; secreted in plasma (at protein level). .

Applications	ELISA
Research Area	Cardiovascular,Complement,Cytokines,G Protein Signaling,Immunology,Innate Immunity,Lipids/Lipoproteins,Macrophage/Inflammation,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001071	EK1544	96 wells/kit	$499		Add to Cart

Human SAA/SAA1 ELISA Kit PicoKine®

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