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Home / ELISA Kit

Human S100 calcium binding protein A9/calgranulin B (S100A9) ELISA Kit

SKU : BHE13702975

S100 calcium binding protein A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. This contributes to creating an overall immunosuppressive microenvironemnt that may contribute to the inability of a protective or therapeutic cellular immune response to be generated by the tumor-bearing host.
Outside of malignancy, S100A9 in association with its dimerization partner, S100A8 (MRP8 or calgranulin A) signals for lymphocyte recruitment in sites of inflammation. S100A9/A8 (synonyma: Calgranulin A/B; Calprotectin) are also regarded as marker proteins for a number of inflammatory diseases in humans, especially in rheumatoid arthritis.

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Mfr.No.	KTE60759
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human S100 calcium binding protein A9/calgranulin B (S100A9) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A9.
Alternative Names	S100A9; 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); calgranulin B
Detection Method	Colorimetric
Kit Components	Human S100 calcium binding protein A9/calgranulin B microplate Human S100 calcium binding protein A9/calgranulin B standard Human S100 calcium binding protein A9/calgranulin B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human S100 calcium binding protein A9/calgranulin B (S100A9) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A9. No significant cross-reactivity or interference between Human S100A9 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	S100 calcium binding protein A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. This contributes to creating an overall immunosuppressive microenvironemnt that may contribute to the inability of a protective or therapeutic cellular immune response to be generated by the tumor-bearing host. Outside of malignancy, S100A9 in association with its dimerization partner, S100A8 (MRP8 or calgranulin A) signals for lymphocyte recruitment in sites of inflammation. S100A9/A8 (synonyma: Calgranulin A/B; Calprotectin) are also regarded as marker proteins for a number of inflammatory diseases in humans, especially in rheumatoid arthritis.
Notes	This Human S100 calcium binding protein A9/calgranulin B (S100A9) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A9 in samples. An antibody specific for S100A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6280

Applications	ELISA
Applications Notes	This Human S100 calcium binding protein A9/calgranulin B (S100A9) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A9 in samples. An antibody specific for S100A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702975	KTE60759	48 T	$339		Add to Cart
BHE13702975	KTE60759	96 T	$589		Add to Cart
BHE13702975	KTE60759	96 Tx5	$2629		Add to Cart
BHE13702975	KTE60759	96 Tx50	$24569		Add to Cart

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