Human S100 calcium binding protein A8/calgranulin A,S100A8 ELISA Kit

Mfr.No.

CSB-E11833h

Sensitivity

0.31 ng/mL

Detection Range

1.25 ng/mL-80 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

S100 calcium binding protein A8, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, OTTHUMP00000015330, S100 calcium-binding protein A8, S100 calcium-binding protein A8 (calgranulin A), calgranulin A, cystic fibrosis ant

Recovery

The recovery of mouse IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-101
EDTA plasma (n=4)	101	90-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-103
1:2	Average %	91
	Range %	83-95
1:4	Average %	94
	Range %	86-98
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.796	1.829	1.813	1.597
100	1.346	1.368	1.357	1.141
50	0.930	0.976	0.953	0.737
25	0.712	0.734	0.723	0.507
12.5	0.515	0.527	0.521	0.305
6.25	0.407	0.423	0.415	0.199
3.12	0.311	0.319	0.315	0.099
0	0.218	0.214	0.216	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P05109

Applications	ELISA
Research Area	S100A8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504207	CSB-E11833h	96T	$695	Add to Cart
BHE10504207	CSB-E11833h	5x96T	$3336	Add to Cart
BHE10504207	CSB-E11833h	10x96T	$6602.50	Add to Cart

Human S100 calcium binding protein A8/calgranulin A,S100A8 ELISA Kit

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