Human S100 calcium-binding protein A8/A9 complex(S100A8/A9)Elisa kit

Mfr.No.

CSB-E12149h

Alternative Names

S100 calcium-binding protein A8/A9 complex(S100A8/A9)Elisa kit

Reactivity

Human

Detection Range

1.56 ng/mL-100 ng/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse CLCF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	91	85-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLCF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	89
	Range %	85-93
1:4	Average %	103
	Range %	98-109
1:8	Average %	85
	Range %	80-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.401	2.293	2.347	2.204
750	1.927	1.876	1.902	1.759
375	1.315	1.361	1.338	1.195
187.5	0.869	0.837	0.853	0.710
94	0.574	0.584	0.579	0.436
47	0.338	0.319	0.329	0.186
23.5	0.232	0.245	0.239	0.096
0	0.146	0.140	0.143	?

Species

Homo sapiens

Sensitivity

0.39 ng/mL

Limit Of Detection

450 nm

Antigen	S100 calcium-binding protein A8/A9 complex（S100A8/A9）Elisa kit

Applications

ELISA

Research Area

S100A8, A9

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Granulocytic myeloid-derived suppressor cells are cryosensitive and their frequency does not correlate with serum concentrations of colony-stimulating factors in head Trellakis S et al,Innate Immun,2013 19(3):328-36. IL-10 Promotes Secretion of S100A8/A9 from Human Monocytes Trough an Inclusion in Plasma Membranes Diercks BP et al,Scand J Immunol,2013 Feb;77(2):169-70. Can Wound Exudate from Venous Leg Ulcers Measure Wound Pain Status?: A Pilot Study Taichi Goto .et al,PLoS ONE,Dec 9 Serum Calprotectin and Prealbumin Levels among Ovarian Cancer Patients Aged 30 - 45 Years Anastasia Petsa.et al,Open Journal of Obstetrics and Gynecology,Mar 22 Comparison of clinical parameters, microbiological effects and calprotectin counts in gingival crevicular fluid between Er: YAG laser and conventional periodontal Wang, Yue MD.et al,Medicine,Dec

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SKU	Supplier No.	Size	Your price
BHE10504206	CSB-E12149h	96T	$790	Add to Cart
BHE10504206	CSB-E12149h	5x96T	$3792	Add to Cart
BHE10504206	CSB-E12149h	10x96T	$7505	Add to Cart

Human S100 calcium-binding protein A8/A9 complex(S100A8/A9)Elisa kit

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