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Home / ELISA Kit

Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit

SKU : BHE13702862

S-phase kinase-associated protein 2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase.

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Mfr.No.	KTE60646
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP2.
Alternative Names	SKP2; FBL1; FBXL1; FLB1; MGC1366; CDK2/cyclin A-associated protein p45; S-phase kinase-associated protein 2
Detection Method	Colorimetric
Kit Components	Human S-phase kinase-associated protein 2 microplate Human S-phase kinase-associated protein 2 standard Human S-phase kinase-associated protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SKP2. No significant cross-reactivity or interference between Human SKP2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	S-phase kinase-associated protein 2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase.
Notes	This Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP2 in samples. An antibody specific for SKP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6502

Applications	ELISA
Applications Notes	This Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP2 in samples. An antibody specific for SKP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702862	KTE60646	48 T	$339		Add to Cart
BHE13702862	KTE60646	96 T	$589		Add to Cart
BHE13702862	KTE60646	96 Tx5	$2629		Add to Cart
BHE13702862	KTE60646	96 Tx50	$24569		Add to Cart

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