Matrices listed below were spiked with certain level of RXRa and the recovery rates were calculated by comparing the measured value to the expected amount of RXRa in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
98
EDTA plasma(n=5)
85-94
92
heparin plasma(n=5)
93-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RXRa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
96-102%
85-101%
85-98%
EDTA plasma(n=5)
83-100%
89-94%
84-91%
heparin plasma(n=5)
84-94%
83-100%
80-97%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
P19793
Applications
ELISA
Applications Notes
RXRa ELISA Kit allows for the in vitro quantitative determination of RXRa concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RXRa (Retinoid X Receptor Alpha) ELISA Kit