Matrices listed below were spiked with certain level of RPRM and the recovery rates were calculated by comparing the measured value to the expected amount of RPRM in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-103
96
EDTA plasma(n=5)
88-99
93
heparin plasma(n=5)
86-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RPRM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
RPRM ELISA Kit allows for the in vitro quantitative determination of RPRM concentrations in serum, plasma, tissue homogenates and other biological fluids.