Matrices listed below were spiked with certain level of RPIA and the recovery rates were calculated by comparing the measured value to the expected amount of RPIA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-95
92
EDTA plasma(n=5)
87-100
92
heparin plasma(n=5)
85-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RPIA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
RPIA ELISA Kit allows for the in vitro quantitative determination of RPIA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RPIA(Ribose-5-phosphate isomerase) ELISA Kit