Human Roundabout Homolog 4 - ROBO4 ELISA Kit

Mfr.No.

E6106Hu

Sensitivity

9.26ng/L

Detection Range

20-7000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Magic roundabout;ROBO 4;ROBO4;Roundabout homolog 4

Molecular Weight

107457

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6106Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1998.4	72.28	3.6
Intra-Assay	2	18	890.1	56.93	6.4
Intra-Assay	3	18	1564.6	105.13	6.7

Background

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity). Involved in the maintenance of endothelial barrier organization and function (PubMed: 30455415).

Protocol

These standard curves of E6106Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	2.075	2.223	2.194
	2.37
2000ng/L	1.208	1.228	1.199
	1.248
1000ng/L	0.815	0.831	0.802
	0.848
500ng/L	0.487	0.445	0.417
	0.404
250ng/L	0.267	0.218	0.189
	0.169
0ng/L	0.031	0.029	0
	0.026

Gene ID	54538
UniProt ID	Q8WZ75

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107468	E6106Hu	48T	$320		Add to Cart
BHE12107468	E6106Hu	96T	$458		Add to Cart

Human Roundabout Homolog 4 - ROBO4 ELISA Kit

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