Human Roundabout homolog 4(ROBO4) ELISA kit

Mfr.No.

CSB-EL020057HU

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

roundabout homolog 4, magic roundabout (Drosophila), ECSM4, FLJ20798, MGC133352, MGC133353, MRB, roundabout homolog 4, magic roundabout

Recovery

The recovery of dog IL13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-112
EDTA plasma (n=4)	85	80-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-102
1:2	Average %	92
	Range %	88-96
1:4	Average %	90
	Range %	86-94
1:8	Average %	87
	Range %	82-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q8WZ75

Applications	ELISA
Research Area	ROBO4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504195	CSB-EL020057HU	96T	$695	Add to Cart
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