Human Roundabout homolog 1(ROBO1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL020054HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

roundabout, axon guidance receptor, homolog 1 (Drosophila), DUTT1, FLJ21882, MGC131599, MGC133277, SAX3, roundabout 1

Recovery

The recovery of pig IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-104
EDTA plasma (n=4)	98	90-112

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-99
1:2	Average %	95
	Range %	90-104
1:4	Average %	98
	Range %	92-110
1:8	Average %	95
	Range %	85-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9Y6N7

Applications	ELISA
Research Area	ROBO1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504194	CSB-EL020054HU	96T	$695	Add to Cart
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