Human Rotatin - RTTN ELISA Kit

Mfr.No.

E6104Hu

Sensitivity

3.12ng/L

Detection Range

7-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

MSSP;Rotatin;RTTN

Molecular Weight

248630

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6104Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	196.11	8.36	4.26
Intra-Assay	2	18	378.6	14.23	3.76
Intra-Assay	3	18	111.03	5.59	5.04

Background

Involved in the genetic cascade that goves left-right specification. Plays a role in the maintenance of a normal ciliary structure. Required for correct asymmetric expression of NODAL, LEFTY and PITX2.

Protocol

These standard curves of E6104Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.027	2.11	2.079
	2.193
400ng/L	1.347	1.451	1.42
	1.556
200ng/L	0.733	0.719	0.688
	0.705
100ng/L	0.373	0.352	0.322
	0.332
50ng/L	0.216	0.178	0.147
	0.14
0ng/L	0.037	0.031	0
	0.025

Gene ID	25914
UniProt ID	Q86VV8

Research Area	Developmental Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107466	E6104Hu	48T	$320		Add to Cart
BHE12107466	E6104Hu	96T	$458		Add to Cart

Human Rotatin - RTTN ELISA Kit

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