Human Rotatin - RTTN ELISA Kit

Mfr.No.

E6104Hu

Alternative Names

MSSP;Rotatin;RTTN

Reactivity

Human

Detection Range

7-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

248630

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.12ng/L

Background

Involved in the genetic cascade that governs left-right specification. Plays a role in the maintenance of a normal ciliary structure. Required for correct asymmetric expression of NODAL, LEFTY and PITX2.

Gene ID	25914
UniProt ID	Q86VV8
Antigen	RTTN

Research Area

Developmental Biology

Protocol

These standard curves of E6104Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.027	2.11	2.079
	2.193
400ng/L	1.347	1.451	1.42
	1.556
200ng/L	0.733	0.719	0.688
	0.705
100ng/L	0.373	0.352	0.322
	0.332
50ng/L	0.216	0.178	0.147
	0.14
0ng/L	0.037	0.031	0
	0.025

Precision

We measured random samples of E6104Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	196.11	8.36	4.26
Intra-Assay	2	18	378.6	14.23	3.76
Intra-Assay	3	18	111.03	5.59	5.04

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107466	E6104Hu	48T	$320		Add to Cart
BHE12107466	E6104Hu	96T	$458		Add to Cart

Human Rotatin - RTTN ELISA Kit

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