Matrices listed below were spiked with certain level of RNH1 and the recovery rates were calculated by comparing the measured value to the expected amount of RNH1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
94
EDTA plasma(n=5)
88-102
96
heparin plasma(n=5)
85-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RNH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-100%
90-100%
92-98%
EDTA plasma(n=5)
87-96%
86-101%
85-100%
heparin plasma(n=5)
88-99%
86-100%
85-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P13489
Applications
ELISA
Applications Notes
RNH1 ELISA Kit allows for the in vitro quantitative determination of RNH1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Reviews of Human RNH1(Ribonuclease inhibitor) ELISA Kit