Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of RNASEL and the recovery rates were calculated by comparing the measured value to the expected amount of RNASEL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-100
94
EDTA plasma(n=5)
87-103
92
heparin plasma(n=5)
90-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RNASEL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-94%
86-93%
95-103%
EDTA plasma(n=5)
82-101%
82-94%
82-96%
heparin plasma(n=5)
86-100%
81-100%
82-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q05823
Applications
ELISA
Applications Notes
RNASEL(2-5A-dependent ribonuclease) ELISA Kit allows for the in vitro quantitative determination of RNASEL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RNASEL (2-5A-dependent ribonuclease) ELISA Kit