Human Rna Polymerase Ii Subunit A C-terminal Domain Phosphatase

Mfr.No.

E6507Hu

Sensitivity

9.32ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CCFDN;CTDP1;FCP1;RNA polymerase II subunit A C-terminal domain phosphatase;TFIIF-associating CTD phosphatase

Molecular Weight

104399

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6507Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1726.61	63.41	3.67
Intra-Assay	2	18	429.17	25.73	6
Intra-Assay	3	18	865.94	44.9	5.19

Background

Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation.

Protocol

These standard curves of E6507Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.197	2.151	2.103
	2.104
1200ng/L	1.336	1.384	1.336
	1.432
600ng/L	0.769	0.831	0.782
	0.892
300ng/L	0.549	0.509	0.46
	0.468
150ng/L	0.275	0.254	0.206
	0.232
0ng/L	0.048	0.048	0
	0.048

Gene ID	9150
UniProt ID	Q9Y5B0

Research Area	Signal Transduction; Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115381	E6507Hu	48T	$320		Add to Cart
BHE12115381	E6507Hu	96T	$458		Add to Cart

Human Rna Polymerase Ii Subunit A C-terminal Domain Phosphatase - CTDP1 ELISA Kit

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