Human RNA-binding protein FUS (FUS/TLS) ELISA kit

Human RNA-binding protein FUS (FUS/TLS) ELISA kit

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Mfr.No.

CSB-E17376h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

fusion (involved in t(12;16) in malignant liposarcoma), ALS6, FUS1, HNRNPP2, POMP75, TLS, 75 kDa DNA-pairing protein, RNA-binding protein FUS, fus-like protein, fusion gene in myxoid liposarcoma, heterogeneous nuclear ribonucleoprotein P2, oncogene TLS, transl

Recovery

The recovery of human ENO1/ENO1L1/MBPB1/MPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-100
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ENO1/ENO1L1/MBPB1/MPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-97
1:2	Average %	92
	Range %	88-96
1:4	Average %	94
	Range %	89-98
1:8	Average %	100
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.321	2.319	2.320	2.225
10	1.982	1.967	1.975	1.880
5	1.470	1.576	1.523	1.428
2.5	1.003	0.995	0.999	0.904
1.25	0.638	0.652	0.645	0.550
0.625	0.380	0.391	0.386	0.291
0.312	0.293	0.299	0.296	0.201
0	0.093	0.097	0.095	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P35637

Applications	ELISA
Research Area	FUS

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504190	CSB-E17376h	96T	$790	Add to Cart
BHE10504190	CSB-E17376h	5x96T	$3792	Add to Cart
BHE10504190	CSB-E17376h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.