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Home / ELISA Kit

Human RNA-binding protein 8A (RBM8A) ELISA Kit

SKU : BHE13703037

RBM8 encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events.
Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.

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Mfr.No.	KTE60821
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human RNA-binding protein 8A (RBM8A) ELISA Kit employs a two-site sandwich ELISA to quantitate RBM8A.
Alternative Names	RBM8A; BOV-1A; BOV-1B; BOV-1C; MDS014; RBM8; RBM8B; Y14; ZNRP; ZRNP1; RNA binding motif protein 8B; binder of OVCA1-1; ribonucleoprotein RBM8
Detection Method	Colorimetric
Kit Components	Human RNA-binding protein 8A microplate Human RNA-binding protein 8A standard Human RNA-binding protein 8A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human RNA-binding protein 8A (RBM8A) ELISA Kit has high sensitivity and excellent specificity for detection of Human RBM8A. No significant cross-reactivity or interference between Human RBM8A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RBM8 encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.
Notes	This Human RNA-binding protein 8A (RBM8A) ELISA Kit employs a two-site sandwich ELISA to quantitate RBM8A in samples. An antibody specific for RBM8A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBM8A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM8A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM8A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9939

Applications	ELISA
Applications Notes	This Human RNA-binding protein 8A (RBM8A) ELISA Kit employs a two-site sandwich ELISA to quantitate RBM8A in samples. An antibody specific for RBM8A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBM8A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBM8A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBM8A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703037	KTE60821	48 T	$339		Add to Cart
BHE13703037	KTE60821	96 T	$589		Add to Cart
BHE13703037	KTE60821	96 Tx5	$2629		Add to Cart
BHE13703037	KTE60821	96 Tx50	$24569		Add to Cart

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