Matrices listed below were spiked with certain level of RLN2 and the recovery rates were calculated by comparing the measured value to the expected amount of RLN2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-98
94
EDTA plasma(n=5)
87-105
96
heparin plasma(n=5)
87-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RLN2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
90-100%
88-102%
EDTA plasma(n=5)
86-101%
85-101%
83-100%
heparin plasma(n=5)
80-100%
94-98%
81-98%
Sample Type
Other liquid samples, Serum, Cell culture supeatants, Cell lysate, Plasma
RLN2 ELISA Kit allows for the in vitro quantitative determination of RLN2 concentrations in serum, plasma, tissue homogenates and other biological fluids.