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Home / ELISA Kit

Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit

SKU : BHE13703004

Eosinophil-derived neurotoxin is a distinct cationic protein of the eosinophil's large specific granule known primarily for its ability to induce ataxia, paralysis, and central nervous system cellular degeneration in experimental animals (Gordon phenomenon). Rosenberg et al. (1989) isolated a 725-bp cDNA clone for EDN. The open reading frame encodes a 134-amino acid polypeptide with a molecular mass of 15.5 kD and a 27-residue N-terminal hydrophobic leader sequence. The sequence of the mature polypeptide was identical to that reported for human urinary ribonuclease and to the N-terminal sequence of human liver ribonuclease. Similarities to the ribonucleases of pancreas and angiogenin indicate that EDN belongs to the ribonuclease multigene family. This gene is also symbolized RNS2 for ribonuclease 2.

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Mfr.No.	KTE60788
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE2.
Alternative Names	RNASE2; EDN; RNS2;
Detection Method	Colorimetric
Kit Components	Human Ribonuclease, RNase A family 2 microplate Human Ribonuclease, RNase A family 2 standard Human Ribonuclease, RNase A family 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human RNASE2. No significant cross-reactivity or interference between Human RNASE2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Eosinophil-derived neurotoxin is a distinct cationic protein of the eosinophil's large specific granule known primarily for its ability to induce ataxia, paralysis, and central nervous system cellular degeneration in experimental animals (Gordon phenomenon). Rosenberg et al. (1989) isolated a 725-bp cDNA clone for EDN. The open reading frame encodes a 134-amino acid polypeptide with a molecular mass of 15.5 kD and a 27-residue N-terminal hydrophobic leader sequence. The sequence of the mature polypeptide was identical to that reported for human urinary ribonuclease and to the N-terminal sequence of human liver ribonuclease. Similarities to the ribonucleases of pancreas and angiogenin indicate that EDN belongs to the ribonuclease multigene family. This gene is also symbolized RNS2 for ribonuclease 2.
Notes	This Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE2 in samples. An antibody specific for RNASE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRNASE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6036

Applications	ELISA
Applications Notes	This Human Ribonuclease, RNase A family 2 (RNASE2) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE2 in samples. An antibody specific for RNASE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRNASE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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