Matrices listed below were spiked with certain level of RHOA and the recovery rates were calculated by comparing the measured value to the expected amount of RHOA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-101
93
EDTA plasma(n=5)
88-105
97
heparin plasma(n=5)
88-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RHOA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
RHOA ELISA Kit allows for the in vitro quantitative determination of RHOA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human RHOA(Transforming protein RhoA) ELISA Kit