Human Rho Guanine Nucleotide Exchange Factor 7

Mfr.No.

E2517Hu

Sensitivity

5.57ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ARHGEF7;Rho guanine nucleotide exchange factor 37

Molecular Weight

76278

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2517Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	170.26	6.4	3.76
Intra-Assay	2	18	519.33	14.42	2.78
Intra-Assay	3	18	493.74	24.74	5.01

Background

Rho guanine nucleotide exchange factor 7 is a protein that in humans is encoded by the ARHGEF7 gene. ARHGEF7 is commonly known as the p21-activated protein kinase exchange factor alpha (beta-PIX or betaPIX), because it was identified by binding to p21-activated kinase (PAK) and also contains a guanine nucleotide exchange factor domain.

Protocol

These standard curves of E2517Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.221	2.112	2.066
	2.003
600ng/L	1.538	1.463	1.417
	1.388
300ng/L	0.602	0.655	0.609
	0.708
150ng/L	0.335	0.344	0.298
	0.352
75ng/L	0.2	0.165	0.119
	0.13
0ng/L	0.034	0.046	0
	0.059

Gene ID	389337
UniProt ID	A1IGU5

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104055	E2517Hu	48T	$320		Add to Cart
BHE12104055	E2517Hu	96T	$458		Add to Cart

Human Rho Guanine Nucleotide Exchange Factor 7 - ARHGEF7 ELISA Kit

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