Human Rho Guanine Nucleotide Exchange Factor 4

Mfr.No.

E3237Hu

Sensitivity

3.14ng/L

Detection Range

5-1000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

APC-stimulated guanine nucleotide exchange factor 1;ARHGEF4;ASEF;ASEF1;GEF4;Rho guanine nucleotide exchange factor 4;SMIM39;STM6

Molecular Weight

79067

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3237Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	133.18	7.61	5.71
Intra-Assay	2	18	332.05	10.07	3.03
Intra-Assay	3	18	244.38	13.11	5.37

Background

Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.

Protocol

These standard curves of E3237Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.63	2.643	2.599
	2.657
320ng/L	1.371	1.384	1.34
	1.398
160ng/L	0.866	0.818	0.773
	0.77
80ng/L	0.532	0.481	0.436
	0.43
40ng/L	0.258	0.257	0.212
	0.256
0ng/L	0.06	0.045	0
	0.029

Gene ID	50649
UniProt ID	Q9NR80

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104766	E3237Hu	48T	$320		Add to Cart
BHE12104766	E3237Hu	96T	$458		Add to Cart

Human Rho Guanine Nucleotide Exchange Factor 4 - ARHGEF4 ELISA Kit

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