Human Rho Gtpase-activating Protein 22

Mfr.No.

E6091Hu

Alternative Names

ARHGAP 22;ARHGAP22;Rho GTPase-activating protein 22;Rho-type GTPase-activating protein 22

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

76779

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.91ng/L

Background

Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter (By similarity).

Gene ID	58504
UniProt ID	Q7Z5H3
Antigen	ARHGAP22

Research Area

Others

Protocol

These standard curves of E6091Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.272	2.288	2.224
	2.304
600ng/L	1.376	1.363	1.299
	1.35
300ng/L	0.863	0.822	0.757
	0.78
150ng/L	0.47	0.504	0.439
	0.538
75ng/L	0.279	0.3	0.236
	0.321
0ng/L	0.051	0.064	0
	0.078

Precision

We measured random samples of E6091Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	204.7	11.89	5.8
Intra-Assay	2	18	384.4	21.7	5.6
Intra-Assay	3	18	772.6	30.16	3.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107453	E6091Hu	48T	$320		Add to Cart
BHE12107453	E6091Hu	96T	$458		Add to Cart

Human Rho Gtpase-activating Protein 22 - ARHGAP22 ELISA Kit

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