Human Rho-associated coiled-coil containing protein kinase 2(Rock-2) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14993h

Alternative Names

Rho-associated, coiled-coil containing protein kinase 2, KIAA0619

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-95
EDTA plasma (n=4)	104	91-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-93
1:2	Average %	111
	Range %	99-114
1:4	Average %	90
	Range %	83-94
1:8	Average %	92
	Range %	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	O75116
Antigen	Rho-associated, coiled-coil containing protein kinase 2

Applications

ELISA

Research Area

ROCK2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10504169	CSB-E14993h	96T	$790	Add to Cart
BHE10504169	CSB-E14993h	5x96T	$3792	Add to Cart
BHE10504169	CSB-E14993h	10x96T	$7505	Add to Cart