Human RHEB(Ras homolog enriched in brain) ELISA Kit
Alternative Names
GTP-binding protein Rheb ELISA Kit, Ras homolog enriched in brain ELISA Kit, RHEB ELISA Kit, RHEB2 ELISA Kit
Reactivity
Human
Detection Method
Double Antibody
Detection Range
0.156-10ng/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of RHEB and the recovery rates were calculated by comparing the measured value to the expected amount of RHEB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-99
95
EDTA plasma(n=5)
98-103
100
heparin plasma(n=5)
86-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RHEB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-105%
87-99%
93-101%
EDTA plasma(n=5)
87-96%
83-101%
82-99%
heparin plasma(n=5)
81-90%
82-95%
82-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q15382
Applications
ELISA
Applications Notes
RHEB ELISA Kit allows for the in vitro quantitative determination of RHEB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
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