Matrices listed below were spiked with certain level of RGN and the recovery rates were calculated by comparing the measured value to the expected amount of RGN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
96-103
100
EDTA plasma(n=5)
87-105
100
heparin plasma(n=5)
85-104
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RGN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
RGN/Regucalcin(RC)/Gluconolactonase(GNL)/Senescence marker protein 30(SMP-30)/
Notes
For Research Use Only
UniProt ID
Q15493
Antigen
Rgn
Applications
ELISA
Applications Notes
RGN ELISA Kit allows for the in vitro quantitative determination of RGN concentrations in serum, plasma, tissue homogenates and other biological fluids.